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Improve sensitivity for rare targets with digital pooling

Rare-target detection is critical for several research applications. The limit of detection (LOD) for a digital PCR (dPCR) reaction depends on several factors, including:

  • Target concentration—samples with low concentration may require testing more sample volume to capture ultra-rare variants
  • Volume of sample analysed per dPCR reaction—some dPCR platforms waste 40% or more of the input sample, increasing the risk of missing rare targets
  • Number of dPCR microchambers assessed—increasing the number of microchambers tested increases the sample volume tested and in turn improves the LOD for rare targets

With digitisation efficiency of greater than 99% into over 20,000 dPCR microchambers and minimal reagent waste of under 5%, the Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR System is well suited to maximise the volume of sample tested per dPCR reaction.

The digital pooling feature of the QuantStudio Absolute Q system provides a simple and flexible tool that enables users to digitally pool multiple arrays of an Applied Biosystems™ QuantStudio™ MAP16 Digital PCR Plate to flexibly analyse up to 320,000 individual microchambers together as one.

Link: Thermo Fisher Scientific

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